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Similarly, FLP- FRT is effective in producing SSI clones at high efficiency in plants [ 12, 13].
If these populations were intermediates on the way to the dominance of QS49 (red) seen in the high relatedness populations, we would expect to see even fewer of these loser clones at high relatedness, but in fact we see many more (mean summed population frequency 0.093).
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B22a1 could also stimulate the 3D4-1.2 3D4-1.2t high clonentration but failed to activate CII-specific clones not expressing thighα16/Vβ12 Tconcentrationn.
This could be indicative of positive selection and dissemination of a single nalidixic acid resistant H58 clone at high frequency in Kathmandu.
DOI: http://dx.doi.org/10.7554/eLife.03300.004 The genes in each mutant library were cloned at high efficiency into a bidirectional influenza reverse-genetics plasmid (Hoffmann et al., 2000).
However, the same situation exists in the mouse, where over 30 α-defensin genes were included in the latest assemblies and over 20 different cDNAs were reported, but only six cDNAs have been cloned at high frequencies.
However, one of the strains cheating during fruiting body formation was the most successful clone at high relatedness, indicating that it did not face a trade-off between the ability to cheat other strains and the ability to develop by itself.
Here we demonstrated that highly enriched mesenchymal stem cells (PαS cells) produce high-quality iPS clones at a high frequency.
The library represents 55,256 clones arrayed at high density on positively charged nylon filters (Protéigène).
Furthermore, the dominant clone at high-relatedness was not cheated by the other two that persisted at low relatedness.
Flp-mediated deletion of the 2xTY1 cassette will lead to a frame shift and thus can be used to create loss-of-function clones at very high efficiency, as the flip-out will occur in cis (Hadjieconomou et al. 2011).
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