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The expression of Dkk-1 and PTHrP was not modified in all transfected clones, as compared to the PC-3/Fluc.
In the presence of 10% FCS, the results revealed a non significant difference in the growth capacity of 2 NB8-CXCR4-expressing clones as compared to NB8-E6.
Total bone mineral content measured by pQCT was lower in the tibiae xenografted with the PC-3/Fluc and mock clones, as compared with the sham-operated ones, but significance was reached only for mock 5-xenografted bones.
The mRNA expression of BMP-2,-3,-6 and -7 was not modified in both Nog-KD clones as compared to parental PC-3, while BMP-4 mRNA was undetectable in both parental and Nog-KD cells (not shown).
Here, we observed that the surface of active osteoclasts per trabecular bone area located at the bone/tumor cell interface was increased in animals bearing MDA-B02-ATX clones, as compared to that observed in mice bearing parental or NPP1-expressing tumor cells (Figure 3).
Resulting cell surface CXCR4 expression, as illustrated in Figure 6a, shows that strong and efficient knock-down of CXCR4 was achieved in both N91-shRNA-CS1 and N91-shRNA-CS2 clones as compared to N91-pAB303 control cells (5.3% and 7.7% vs 53% CXCR4 positive cells respectively).
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Regarding the apoptotic rate, administration of TZD had no effect on the HT29 shPPARG clone, as compared to parental cells (Figure S4).
Despite the fact that the mRNA expression of IL-8, known to stimulate osteoclast recruitment, was significantly up-regulated in vitro in the Nog-KD 17 clone, as compared to the Nog-KD 14 clone, there was no obvious difference in the osteoclast number between the bones xenografted with the two Nog-KD clones.
Given the association between repetitive elements and methylation, it is expected that repetitive elements should be less frequent near the start of these reads (i.e., the end of the clone) as compared to the end of the reads (i.e., the clone interior).
Since imatinib alone has little or no measurable effect and does not potentiate the effects of established cytotoxic treatment in BCR ABL-negative cells at therapeutically relevant concentrations, even merely additively acting imatiniBCR ABL-negativebinations as shown in cellsells should exert more selective toxicity on the leukatmic cell clone as compared witherapeutically- orelevantherapy alone.
This is illustrated both by the HMPR libraries, which have substantial enrichment for genic sequences coupled with a very low frequency of LTR retrotransposon sequences, and by the MSLL libraries, for which the incidence of retroelements increases greatly within the methylated interior region of the clone as compared to the end sequences, which are at least terminally unmethylated (Table 1).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com