Sentence examples for clones are rescued from inspiring English sources

Our results are consistent with this since the cell morphology and proliferative defects of scrib mutant clones are rescued by aPKCCAAXDN, however, the mutant cells still undergo JNK-mediated apoptosis and can still cooperate with RasACT in tumourigeneis.

In Bim −/− X Nur77GFP mice, however, a significant number of post-selection thymocytes express high levels of GFP, indicating that high affinity TCR clones are rescued from negative selection in the absence of Bim (Stritesky et al., 2013).

Furthermore, when ft mutant clones are rescued by expression of a ft transgene lacking the Dco phosphorylation sites [Ft p-mut (ftmV); Pan et al., 2013], Dachs levels are also elevated (Fig. 2C,D).

All resulting PCR products were sequenced using the same oligonucleotides, and the pAD plasmids of these yeast clones were rescued in DH5α bacteria.

Individual clones were rescued from randomly selected white plaques and grown in (Luria broth + ampicillin) medium.

Prey plasmids of positive clones were rescued using E. coli KC8 and subjected to DNA sequencing.

(E – H′ ) The loss of mature INPs in btd mutant clones is rescued in pros 17 heterozygous mutant larvae.

After two days incubation at 30 °C, well-grown clones were rescued and diluted to OD600 = 0.3, which was further diluted to 1 5 and 1 25 dilution gradients.

After 10 passages, 15 virus clones were rescued from each supernatant by plaque purification for each virus strain, and the hypervariable regions (nsp2 and ORF5) of the 15 virus clones were amplified for sequencing.

To screen monoclonal phage antibodies, individual clones were rescued by transferring inocula from bacterial colonies to the wells of sterile 96 well tissue culture plates containing 100 μl/well 2x TY A/G.

Furthermore, all FE defects observed in baz (as previously reported by Benton and St Johnston, 2003; Morais-de-Sá et al., 2010) and baz EH747 clones were rescued by overexpression of full length UAS-Baz using the MARCM system (supplementary material Fig. S1).