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Paradoxically, despite the fact that vps25 clones are highly apoptotic (Figure 3B), we originally isolated vps25 mutants based on their ability to suppress apoptosis [21].
Dimorphic processes from male olfactory clones are highly enriched in the ring and the junction.
Moreover, the monomers of centromeric and pericentromeric arrays in BAC/YAC clones are highly diverged, making their mutational analysis impossible.
Thus, the efficiency of the reverse transcriptase reaction at amplifying longer templates might actually be the major concern where larger clones are highly desirable in a library.
While the U3 snoRNA and U5 snRNA sequences in the two clones are highly similar, the size (255 and 160 bp) and sequence of the U3 snoRNA-U5 snRNA intergenic spacers are very different (see below).
These clones are highly effective in plasmid transfer across bacteria and are known to carry other plasmid-based antimicrobial drug resistance genes such as those that encode resistance to trimethoprim/sulfamethoxazole, aminoglycosides, and fluoroquinolones (6 ).
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The cytogenetic properties of individual clones were highly heterogeneous and chromosome instability was observed in both spontaneous and radiation-induced mutants.
Deduced amino-acid sequences from seven BAC clones were highly conserved with the rice, maize, and sorghum lax-barrenstalk1orthogenes.
Second, only one of the two major disclosed K2 clones was highly virulent to mice.
As shown in Figure 1B, the majority of shRNA clones were highly resistant to HIV-1 replication.
After two rounds of sorting, >78% of the enriched clones were highly fluorescent in all three libraries (Figure 2B).
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