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As shown in Table 1, the candidate external RNA clones are either synthesized from unique sequences (i.e., artificial) or are derived from genes in several non-mammalian species.
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The remaining 14 antibody clones were either bi-specific (CK5 CK10) or mono-specific (CK11 CK18).
This process was repeated iteratively until all clones were either accepted or rejected.
Clones were either positive or negative for the two markers, which allowed their classification as myogenic or nonmyogenic, respectively, even when myotubes were not apparent.
Clones were either positive or negative for the two markers, which allowed us to classify clones as myogenic or nonmyogenic, respectively, even when myotubes were not apparent [9], [11].
Zeocin resistant cell clones were either mock treated or treated with 1 µg/mL tetracycline, 1 µg/mL tetracycline and 2.5 mM sodium butyrate for 48 hours to induce OR131-2 protexpressionsion.
The other six clones were either B genome or D genome lacking the mutation.
Promoter entry clones were either retrieved from the C. elegans Promoterome library or generated ab initio by Gateway cloning.
Fig. 3: We have noted some errors in the clone scoring in fig. 3; some of the clones were either misplaced or were missing from the dataset presented.
In this work, yeast clones were either referred as P. pastoris/pPGKΔ3 α/L1 or P. pastoris/pPGKΔ3/L1, depending on the vector that was used.
The Entry clones were either obtained via BP-reaction in pDONR201 or pDONR207 or through TOPO-reaction using the pENTR/D-TOPO vector (CTR1, AHK5; all vectors Invitrogen).
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