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The isolation of five TRG1 BAC clones and two TRG2 BAC clones, their sub-cloning in plasmid vectors and the sequencing of their inserts allowed us to obtain contiguous genomic sequences spanning 158.8 Kb for TRG1 [available in GEDI for GenBank/EMBL/DDBJ and IMGT/LIGM-DB: DQ992075] and 95.0 Kb for TRG2 [available in GEDI for GenBank/EMBL/DDBJ and IMGT/LIGM-DB: DQ992074].
Three replicates of three types of planting material (stakes (0.5 m), wands (1 m) and poles (3 m) of each of two poplar clones and two willow clones (Table 1) were established in a field trial near Gisborne (Figure 1) in 2009.
Four heterozygous ES cell clones were identified from screening of 137 clones, and two of these clones were used to generate conditional TANK1 knockout mice.
Two independent experiments were performed in triplicate using two mock clones and two A16 clones.
Two prototypic A16 clones and two prototypic mock clones were used in this study.
From 2 × 10 primary transformants, 18 were encoding true positive clones and two of them encoded vimentin.
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Three newer collected Polish common osier clones, and three clones from Swedish cultivars of common osier, were also used in this study.
All of the EcoRI X clones and three of the EcoRI c clones contained a 30-base-pair (bp) sequence which was directly repeated at the ends of the MCMV genome.
Twenty Vα2+, CD4+ clones and twenty Vα2−, CD4− clones were selected and used for further studies.
Among the sequenced clones, the three major genotypes again were well represented with 51 VT-like clones, twelve T30-like clones, and six T36-like clones.
Cluster B contained the sequences of six clones and one xylanase directly cloned from an uncultured human gut bacterium.
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