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To obtain ES cell clones that carry the PD deletion, we introduced Cre-recombinase into 2loxP clones and selected for HATR, neoS and puroS ES cell colonies.
We checked 12 Tn5-carrying clones originating from the HooFos09, HylFos15, and NomFos1B3 clones, and selected one in which Tn5 had been inserted at a position 10 16 kb distant from one end of its AS array.
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The H3N2-specific VHH genes were cloned and selected from an immunized dromedary.
To test this hypothesis, we transfected Darpp-32 cDNA into the Herceptin-resistant SK.tDp.A clone and selected for zeocin-resistant clones.
We have successfully cloned and selected a scFv specific for noxious CD peptides.
The cells were single cell cloned and selected for Sn expressing CHO and PK15 cells with geneticine (200 μg/ml, GIBCO).
Cells were replated on DMEM+10% FBS (fetal bovine serum) and clones grown and selected by morphology [17].
Each amplicon was subsequently cloned into the modified pDrive cloning vector and selected by PCR colony screening.
The remaining sequences were aligned with clone sequences and selected BLASTn matches using MUSCLE v3.8.31 [44].
The recombinant maxi- and mini-EBV plasmids were separately introduced into the mESC clone Bruce4 EBNandC1 and selected with G418.
We checked 10 clones by sequencing and selected the mutant clones D15 and D19.
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