Sentence examples for clones and found from inspiring English sources

To exclude off-target effect of Cas9, we did sequencing work with the 5F8 and 2F5 clones and found the same non-targeted sequences in predicted top4-scored off-target sites (3 mismatches) as WT NB4 cells.

Restriction digestion analysis was carried out to determine the length of the insert of the positive clones and found insert length in the range of 38 to 41 Kb (Additional file 1: Figure S1D).

This is similar to results obtained by Kabelitz and colleagues, who injected multiple doses of GDTc clones and found small numbers remaining in peritoneal exudates and spleen cells in SCID mice up to 30 days post-injection [47].

To further confirm the functional requirement of kinesin-mediated transport, we generated Klc8e×94 MARCM clones, and found that loss of the Klc activity caused disintegration of the axonal bundles and aberrant dendritic overshooting (Fig. S7A F).

Since Abd-B is common target of PH in both the ovary and wing disc, we induced Abd-B overexpression clones and found that these are round with smooth borders, though not efficiently expulsed from the epithelial layer.

We sequenced six F12A10.3 clones and found two slightly different splicing patterns, both different from Genefinder and Intronerator predictions.

We examined the localisation of GFP-tagged FbxL7 expressed in clones and find that FbxL7-GFP localises to apical cell-cell junctions (Fig. 4A).

Genes encode for Chi25 and Chi35 have been cloned and found to be arranged in tandem.

The growth arrest-specific 2 (GAS2) was cloned and found to be upregulated in the feces of recurrent CRC patients.

The gene that encodes the 73-kilodalton polypeptide, designated TFB1, was cloned and found to be essential for cell growth.

The SKC1 gene was subsequently cloned and found to encode a sodium transporter that helps control K+ homeostasis under salt stress (Ren et al. 2005).