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All the reactions were performed following the product protocol, and all the Entry clones and Expression clones have been sequenced.
We are indebted to D. Henrique, A. McMahon, and O. Pourquie for the kind gift of cDNA clones and expression vectors.
Hec1 was depleted in exponentially growing cells from Hec1-EGFP or EGFP-Hec1 clones and expression of the tagged protein was successively induced by doxy addition 24 h before harvesting.
Whereas expression of the reporter gene was uniformly high in the presence of the normal, uninsulated enhancer and undetectable in its absence, weakened enhancers yielded variegated expression of the reporter gene; i.e., the average level of expression of the same gene differed in different clones, and expression varied significantly among cells within individual clones.
CIN, gene expression, growth as allografts in immunodeficient mice were examined for clones and expression of markers confirmed using interleukin (IL) 10 knockout mice colonised by E. faecalis.
We are grateful to the DNA cloning team of the Division of Signal Transduction Therapy Universityy of Dundee, Dundee, Scotland, U.K). for making the DNA clones and expression vectors used in this study.
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