Because of the limitations of the TA and Gateway cloning systems, most laboratories still use classic restriction enzyme cloning.
Such site-specific recombination based cloning systems have been successfully employed for building a number of clone sets [18], [20] [22], [40] [42].
The Flp double cross system has advantages over currently available cloning systems that use site-specific recombinases or topoisomerase.
An in vitro baculovirus cloning system has been developed for direct cloning of foreign DNA into baculovirus genomes.
The yeast two-hybrid interaction cloning system has now been used to identify additional proteins from Aplysia that interact directly with VAMP.
Products were cloned using the TOPO TA cloning system following instructions of the manufacturer (Invitrogen).
All other ORFs were cloned using the Gateway® cloning system into the pDESTsmg vector [18].
A comprehensive gene collection for S. oneidensis was constructed using the lambda recombinase (Gateway) cloning system.
The pYUB1049 vector has been also converted to a Gateway® cloning system compatible vector, pDESTsmg [18].
PCR products were cloned using half reactions of the TOPO TA cloning system (Invitrogen) with vector pCR 2.1.
