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The gene of AACT was cloned successfully from E. helioscopia, and named as EhAACT.
Fifteen hybridomas produced high affinity antibodies and were cloned successfully.
The promoter regions of BK-2, CHRM1 and CT-R were cloned successfully.
The Rshtmt gene was cloned successfully and expressed in E. coli cells.
Using this technique, Xenopus laevis and the famous sheep "Dolly" were cloned successfully in 1960s and 1990s, respectively.
Twenty genes (Additional file 1) corresponding to these proteins were cloned successfully according to our EST database (unpublished data), and their expression patterns at 0 and 5-day-old cutting bases were investigated at the mRNA level using qRT-PCR.
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Unique MGC cDNAs, initially generated largely by random cDNA library screening, were used individually as templates to clone successfully 4111 additional ORFs, generating a consolidated collection of 12,212 ORFs representing 10,214 genes.
He said the Japanese cloned it, successfully started a new crop and with the financial rewards "paid for 30 years of research".
In this study, Δ59 SrtA was cloned and successfully expressed in E. coli at first.
One of the identified BVMO-encoding genes was cloned and successfully overexpressed fused to the cofactor regenerating enzyme phosphite dehydrogenase (PTDH).
Most mutant clones in the TAS2R16 library were fully translated (91% of clones were fully expressed at >50% of wild-type levels), were well-expressed on the cell surface (88% of clones successfully trafficked to the cell surface at >50% of wild-type levels), and encoded functional receptors (60% of clones signaled at >50% of wild-type levels).
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