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In other cases, researchers create genomic or complementary DNA (cDNA) libraries to identify interesting genes or to provide cloned material for sequencing.
The PCR amplicons were cloned and sequenced, and the DNA sequence analysis of the cloned material was in agreement with the ARMS results in all but one case.
In locus MS16, a stutter peak of one repeat unit smaller than the dominant peak was observed in the PCR amplification of the cloned material and in the samples.
Moa tbx5 sequences were obtained as ~120 bp overlapping fragments from a number of species either by direct sequencing of PCR products or cloned material (Additional file 1: Figures S3-5, S8).
The numbers provided by NMRC in this table are based on real- time polymerase chain reaction quantification, where a standard curve was produced with cloned material and used to calculate the results of a gene copy/mL quantification of the autoclaved material.
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Of course, while McGrady's website is by no means winning any design awards (and has clone material written all over it), or pushing the envelope technologically, it gets the job done, and, perhaps more importantly, it does so in a way that doesn't stink of brand shilling.
For VH cloning material from 44 donors of the previous described HAL7/8 libraries [ 31] was used in addition to material of 54 new donors.
Dr. Primi cloned the material and then found it in the blood of other AIDS patients but not in otherwise healthy people in Italy.
Kelch motif BnACBPs were cloned from material grown in a semi-winter type area in China, they were the subject to further function analysis; but also, different lines SNP might exist as the material used for the sequencing of B. napus is not the same.
We used unique clone material derived from fully-grown Norway spruce trees with either high or low susceptibility to Heterobasidion spp. as measured in a field trial [ 7].
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