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Additionally, TiPS provide a novel model with which to explore iPSC clone tracking, T-cell development and therapeutic applications of iPSC technology.
Additionally, they retain their characteristic T-cell receptor (TCR) gene rearrangements, a property which could be exploited for iPSC clone tracking and T-cell development studies.
TCR rearrangements may in fact prove advantageous in certain contexts, such as for iPSC clone tracking, as demonstrated by the detection of parent line clonal TCR β chain rearrangements in derivative teratomas (Figure 5).
Each tracked clone had BLAST identifiers matching gene IDs predicted from the re-array spreadsheet, indicating highly accurate clone tracking throughout the process of microarray fabrication.
DOI: http://dx.doi.org/10.7554/eLife.01569.007 Clone tracking demonstrated that the epithelial cells shift during postembryonic development and change their neighbours.
Using this approach for clone tracking, they discovered that epithelial cells shift during postembryonic development, and that cells shift anterior wards.
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Surprisingly, the lethal clone was tracked to a small focus of Gleason 3 disease in the primary tumor, not bulkier, higher-grade disease, or the early pelvic node metastasis [ 56].
We analyze here a systematic clone-tracking study that used a large number of HSPC clones in a transplant and competitive repopulation setting comparable to that used in humans [ 19].
The ensuing host polymorphism leads to clonal interference, differentially reducing the ability of clones to track rapidly changing environments and giving sex a comparative fitness advantage that allows it to compete with cloning on equal terms, or displace it.
JK designed and implemented software for tracking clones and sequences.
By molecular bar coding and tracking individual clones, we found that, although AZA alters the sub-clonal contribution to different lineages, founder clones are not eliminated and continue to drive hematopoiesis even in complete responders.
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