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We cloned NP with these modifications and a C-terminal 6-histidine tag into pET28b, transformed into BL21 Star DE3 (Invitrogen, Grand Island, NY, C6010-03), and grew 1 L of these bacteria to an OD600 of 0.3 0.6 at 37°C.
The extreme sensitivity resulting from these modifications permits cloning of new members of a closely homologous gene family from only one microdissected tissue sample and widens the applicability of tissue microdissection.
However, these modifications did not significantly affect cloning efficiency.
These modifications may reach remarkable extremes.
The new catalyst makes these modifications easier.
"The idea was strong enough to sustain these modifications".
Unfortunately, these modifications sometimes cause problems.
Consider these modifications in turn.
these modifications?
These modifications are.
These modifications were made in Kiel.
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