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An unauthorized clone system known as the Telegames Personal Arcade was also produced, and was able to play games from both the SG-1000 and Coleco's ColecoVision console.
The F857-L mutation was then introduced back to the genome of Vero-adapted IBV by using an infectious clone system based on p65 [17], [17] to test its influence on viral recovery and infectivity.
Introduction of the A159-K160 mutatintointo an infectious IBV clone system blocks the infectivity of the clone, although viral RNA replication and subgenomic mRNA transcription were actively detected.
Introduction of the A159-K160 mutatintointo an infectious IBV clone system showed no infectious virus could be recovered, although viral RNA replication and subgenomic mRNA transcription were detected.
In addition, we noticed LacZ+ germ cell clones with the TIE-DYE clone system, indicating that line 688A also expresses Flp in the germline.
To further investigate the escort cell clones produced by line 688A, we used the multi-color TIE-DYE clone system (Worley et al. 2013).
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For the retro gaming fans out there, these clone systems are making a lot of dreams come true.
The product was cloned with the Invitrogen TA cloning system, and 16 colonies per sample were sequenced with the commercial facility offered by Macrogen (Seoul, Korea).
AcrIIA6 was subcloned using the Gateway cloning system into a bacterial expression vector pETG-20A (with an N-terminal 6His-TRX-tev tag).
An in vitro baculovirus cloning system has been developed for direct cloning of foreign DNA into baculovirus genomes.
These libraries were cloned using a Golden Gate cloning strategy making them usable as standard parts in a modular cloning system.
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