Sentence examples for clone screening from inspiring English sources

Flexi-taq (Promega) was used for routine clone screening.

But isolating recombinant mammalian cell lines for large-scale production remains costly and time-consuming, due to substantial variation and unpredictable stability of expression amongst transfected cells, requiring extensive clone screening to identify suitable high producers.

This strategy reduces pool number and adopts the highly parallel GoldenGate assay for clone screening, and makes high-throughput clone screening possible for large genomes.

Following clone screening, cells expressing the desired plasmid were expanded successively into 6-well plates and 25 cm2 T-flasks.

After transfection of individual sets of TALEN vectors and donor plasmids into HCT116 cells, puromycin was added to facilitate positive single clone screening.

Briefly, the glycoaminoacid GalNAc-α O-Ser (Tn) was synthesized, conjugated with cBSA for mice immunization and with biotinylated alginate for serum titration and anti-Tn clone screening.

In an example of gene targeting, a knock-in mouse was produced from an ES clone screened from 200 G418-resistant colonies generated via M9-assisted lipofection.

Identification of high expression cell clones (screening) was either carried out by multiple rounds of FACS sorting or limited dilution cloning steps.

Of 96 clones screened on solid media, one showed elevated indigo production compared to the parental forms.

Among 63 positive cosmid clones screened using KS probes, only 2 cosmid clones were hybridized with ALS probe and only 3 clones with FkbH probe during the second screening.

Among 513 RNAi clones screened, 21 displayed a synthetic Daf-c (SynDaf) phenotype with sdf-9.