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Each clone (product and entry) can be used as a new entry-clone (Marquardt et al. 2014).
Monoclonal antibodies (mAbs): Specificity Clone Product # Isotype Source Anti-β1 integrin P4C10 MAB1987 IgG1 Chemicon Int.
That report said the risks of animal cruelty were grave enough to keep cloned products off the European market.
Current commercial cloning products are not robust enough to support the assembly of very large or very small genetic elements or a combination of both.
We cloned products that contained sequences of more than one locus.
Cloned products were then sequenced with an Applied Biosystems 3130xl Genetic Analyzer.
Cloned products for the E1 E2 region or NS5A region were sequenced by using vector-based primers KS and SK (Stratagene).
Cloned products for E1-E2 aNS5AS5A regions were sequenced using vector-based primers KS and SK (Stratagene).
Indeed, DNA is mostly spread by the experimenters who can be repeatedly contaminated by previous PCR and cloning products.
The final cloning products were fully sequence confirmed with primers for the T7 promoter and terminator site of pET28a (Novagen primers 69348-3 and 69337-3).
The UCSC genome browser and Sanger miRBase were used to analyze cloned products, to assess microRNA conservation, and for primer design.
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