Individual plants produce several ramets and form small clone patches.
This estimate is comparable to previous findings in the perennial pleurocarp H. splendens where an average of 2.2 and 2.6 clones per patch (1 dm) has been detected [ 49, 50].
Resulting clones were patched onto Chamberlain's defined medium (CDM) and MH containing kanamycin to confirm auxotrophy and loss of antibiotic resistance, respectively.
From the normalized and full-length enriched cDNA library, 13,220 clones were patched for plasmid DNA isolation.
(E ) An example of GFP-, shg 10469 clone without Baz patch (yellow line).
This would mean that the pollen deposited by A. mellifera could represent a high self-pollination rate from other stems of the same clone within a patch.
To check the functionality of the clone, whole-cell patch-clamp measurements of citrate-induced currents were performed.
This study describes an optimized homologous DNA assembly method, termed as multiple patch cloning (MUPAC), for multiple site-directed and saturation mutagenesis.
In this study, we developed an optimized DNA assembly method, termed multiple patch cloning (MUPAC), which could assemble up to 11 DNA fragments to introduce nine site-directed mutations.
Because a count of unit cell) number is unfeasible in this phase, we characterized clone size by the patch length along the axis of the tubule.
