The cloning efficiency was calculated by the formula: colony formation rate = (clone number / inoculation cell number) × 100%.
Each isolated clone was named using a three number code where the first number denoted the isolation number (I); the second number, the tank from where it was isolated (T) and the third, the clone number (C): #I.T.C.
The numbers above the figure correspond to the clone number.
When clone number was used as a continuous variable, the risk of re-infection associated with clone number was OR = 1.29 (95%CI 1.06 1.58, P = 0.013).
Each text file was given a specific clone number, cell line name, and date.
Antibodies are listed in Table 8 with dilution, manufacturer and clone number and secondary antibody.
Twenty-one sequences were obtained (Figure S1) and they are termed G12SD-X, where X is the clone number.
A 125 bp segment of the 5'-untranslated region (UTR) and 2.3 Kbp of the TRR of human PIK3CA were cloned from human bacterial artificial chromosome (BAC, clone number: PR11-245C23).
Second, TAR-cloned BESs were sequenced, and we will refer to these clones either by their TAR clone number or by the BES to which they have been assigned.
Increasing clone number was associated with increasing risk of subsequent exposure to parasites, and the presence of 2 or more clones was associated with more blood stage immunity (i.e. less febrile malaria compared with asymptomatic infection).
(2) The clone number was limited.
