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I see it in people's eyes when I explain that Church's CRISPRs method isn't cloning exactly, more just making a genetically engineered elephant that can handle the cold – this just isn't as emotionally satisfying as the Sooam approach: taking an actual mammoth cell nucleus, DNA intact, and popping that into an elephant egg.
However, it remains to be determined if the BMP expression profile of the various clones exactly matches the BMP profile of the corresponding formed tumors.
The odds are improved by providing each clone with exactly the same living environment that the horse it was cloned from was used to.
In a control experiment we PCR amplified and cloned a clone using exactly the same protocol as described in above.
(C ) Quantitation of the clone showing exactly same number (136) of cells in both red and green clone of twin-spot.
The two longer RNA species of roughly 137 nt (majority of the sequenced R2 clones) and 211 nt (half of sequenced R3 clones) share exactly the same 5' extremity.
For clones with exactly two measurements, s.d.'s of growth rates were estimated using posterior distributions of CCFs.
We set up the cloning reaction exactly the same way as in single-cycle CPEC.
FISH of whole scaphognathite tissues from an individual not used for the preparation of the clone libraries was exactly carried out as previously described [24].
Sequencing results of randomly picked colonies confirmed that the cloning reaction happened exactly as expected, with no mutations at the cloning junctions.
In Greece, the Explanatory Memorandum to the legislation declares that ' [i]t could be...that therapeutic cloning is permitted exactly as in Article 1 paragraph 1 of the Additional Protocol on Cloning'.
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