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"CloneAssistant 1.0" makes gene clone design much easier, and it can be freely downloaded from http://bis.zju.edu.cn/clone.
This data is necessarily heterogeneous in nature, ranging from materials and methods, construct and clone design, protein expression and purification to small molecule screening and protein structure determination information.
Among the 800 BAC clones, 17 spots were omitted from analysis because of collation with the latest genomic data and re-evaluation of clone design.
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Recently, we described a novel vaccination strategy based on iDNA® infectious clone designed to launch a live-attenuated CHIKV vaccine from plasmid DNA in vitro or in vivo.
VM participated in the cloning design.
SWPC performed molecular cloning, design of plasmids, expression and purification of proteins, and design and running LC-MS assays.
SnapGene software (from GSL Biotech, Chicago, IL; available at www.snapgene.com) was used for molecular cloning design.
This blunderbuss approach fails to distinguish between potentially valuable therapeutic cloning, designed to produce stem cells for human therapy, and the more questionable reproductive cloning, designed to produce a human baby.
The full-length EnStef gene was amplified using the rEnStefFWD (5′-CATAcata/tgATGCAAATGGTTGGAGGAATT-3′) and rEnStefREV (5′-GATAc/tcgagTCAAAAATATTCCAAGATATCGTC-3′) expression primers with NdeI/XhoI restriction sites for directional cloning (designed based on the sequence obtained after 5′ RACE PCR).
BlastX search and subsequent phylogenetic analysis showed that one of the positive clones, designed as RGK32, displayed high homology with ketosynthase domains of many type I PKS systems and may belong to the subclass cis-AT PKS group.
This is a study of the behaviors of the naive bootstrap and the Bayesian bootstrap clones designed to approximate the sampling distribution of the Aalen Johansen estimator of a non-homogeneous censored Markov chain.
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