To construct pTCF4-luc, a potential promoter region encompassing ∼1.9 kb upstream of the transcription start site was predicted, acquired from human BAC clone (clone number RP11-57H14 from the library RPCI-11.1; BACPAC Resources, Oakland, CA, USA), and PCR-cloned into pGL-luciferase system.
Owing to a lack of information on the TCF4 promoter region (Duval et al, 2000), a potential promoter region encompassing ∼1.9 kb upstream of the ATG site was acquired from human BAC clone (clone number RP11-57H14 from the library RPCI-11.1; BACPAC Resources, Oakland, CA, USA) and PCR-cloned into pGL-luciferase system.
Ozone exposure decreased leaf, stem, branch, and root dry weight particularly in the O3 sensitive clone (clone 259).
The clone used was a high wood density, high acoustic velocity clone (Clone 35, Forest Genetics CellFor Ltd) produced using somatic embryogenesis.
The addition of CO2 (150 μl1−1 over ambient) to the O3 exposure counteracted the negative impact of O3 only in the O3 tolerant clone (clone 216).
(D) TA cloning results involving a representative homozygous clone (clone 19) show that both alleles of the mouse Clock gene were modified on exon 2 by the CRISPR/Cas9 system.
One BAC clone, clone 6 harbored with the inserter of approximate 115 kb mtDNA sequence, in which more than 10 primers fragments could be amplified, was sequenced and assembled using the Solexa strategy.
That's what you see these days: Hamburger after hamburger, clone clone clone.
One clone (clone 24) was correctly targeted and used to generate knock-in mice (Fig. 1C).
