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The GK2_16 clone achieved a 12% increase in lipid productivity.
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However, after 120 hours, the pBSK-g10-ppk-rpsT clone had better resistance than the pBSK-g10-mt1-rpsT clone; achieving growth saturation in 40 μM Hg.
By contrast, if resources occur in patches that are colonized by one or a few individuals that expand into clonal populations before they disperse again, then the yield that a clone achieves can be decisive for its competitive success (Fig. 2).
The photosynthetic rate and stomatal conductance were assessed and showed 100% successful budding of rootstocks of clone RRIM 600 and clone 4, whereas the other clones achieved 75% success.
The selections were performed in duplicate, with comparable number of surviving clones achieved each time.
None of the KO clones achieved 50%% reduction in RNA levels and one clone displayed a moderate increase (MALAT1_A KO1).
A classifier employing 15 BAC clones achieved the best cross-validation performance, and had an associated validation performance of 91.7%.
Cloning achieves the same end by transferring the genetic material from an existing adult cell (which already carries a full complement of chromosomes) into an egg that has had its half-set of chromosomes removed.
With this setting, a maximum incorporation of clones was achieved, while questionable clone alignments could still be nearly completely removed.
Direct cloning was achieved using either a TOPO T/A cloning kit (Invitrogen, USA) or a pGEM-T Easy (Promega, USA) cloning kit as directed by the manufacturer.
Cloning is achieved by mixing the ss-tailed sense and antisense products with a cloning vector that contains complementary ss-tails under conditions which promote efficient annealing of the fragments.
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