Exact(26)
Our study provides the first analysis on the spatial relation between clonally derived neurons from IPCs and clonal size.
While CD8 cells are thought to play an important role in the control hepatitis C infection, low frequencies of virus-specific cells and high numbers of potential determinants have made it challenging to obtain direct and comprehensive data regarding fine specificity and clonal size of the CD8 cells involved.
Overexpression of the Dakt1 dramatically increases clonal size in wing imaginal disc through an enlargement of the cells [39].
To determine the minimal clonal size that can be detected by SNP-A, dilution studies of trisomy 21 DNA with normal diploid DNA were performed; clones spanning more than 25 50% of the total cell population can be detected.
However, contrary to current models that attribute CD4+ T cell help to CD8+ T cell functionality and not clonal size, our data demonstrate that CD8+ T cells specific for the circumsporozoite protein of P. yoelii that are induced by γ-spz require CD4 help to promote optimal clonal expansion, but not effector function.
No discrepancy was found when Sanger sequencing 15 selected variants over 20% of clonal size.
Similar(34)
Finally clonal sizes were rechecked for frequencies to understand distribution.
Antigenic stimulation induces rapid expansion of antigen-specific T cells that expand to large clonal sizes.
Additionally, it also shows that more IPCs are born during early cortical development (thus giving larger clonal sizes).
In 4 5 day positively marked clones, we observed clonal sizes ranging from typically 2 11 cells (data not shown).
As these clonal sizes can be achieved only when both early and late born IPCs are labeled, this further demonstrates the fidelity of our method.
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