Exact(2)
We used the sIgM loss variant assay to ask if tethered VP16 altered the clonal rate of Vλ sequence diversification.
To ask if tethered HIRA affects the clonal rate of Ig gene diversification, we established independent clonal populations of DT40 PolyLacO-λ HIRA-LacI transfectants by limiting dilution cloning of sIgM+ cells, and following 4 weeks of culture the fraction of sIgM− cells in each population was determined.
Similar(58)
In the TSCE model, it is determined mainly by the net clonal expansion rate of initiated cells (ie, by the difference between proliferation and death/differentiation rates for these cells).
Applying the clonal spread rate to current patches allowed to quantify future distribution patterns.
We found the clonal formation rate of neurospheres from P1 was significantly higher than that of P2 (Fig. 4B).
The increase in patch sizes from the older to the younger photographs was used to calculate a lateral clonal spread rate (0.42 m year−1).
Fertility and viability of the yeast were measured by the sporulation frequency and the clonal growth rate, respectively, for each of the F2 hybrids (Figure 1).
Zbinden et al. [45] provide evidence that resistant D. magna genotype showed a reduced clonal growth rate, but only under stress free conditions (i. e. without interspecific competition).
Comparison of median percentages of sIgM− cells showed that DT40 PolyLacO-λ GFP-LacI-VP16 transfectants exhibited an 8.4-fold increase in clonal diversification rate relative to DT40 PolyLacO-λ GFP-LacI control cells (Figure 2D).
For the clonal growth rate, cells were cultured in 50 ml of YEPD at 30°C for ∼18 hours, with an initial concentration of OD660∼ = 0.05, and the OD660 was measured every two hours using the 2800 UV/VIS Spectrophotometer (UNIC Corp., Shanghai).
We used the sporulation frequency and the clonal growth rate at defined lab conditions as the proxies of yeast ferility and yeast viability, respectively, and observed a much higher growth-based fitness compared to sporulation-based fitness for every single F2 hybrid, strongly supporting that genetic incompatibility affects hybrid sterility more than hybrid viability in yeast.
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