Sentence examples for clonal knockdown from inspiring English sources

Clonal knockdown lines were obtained by limiting dilution.

Virgin females of this genotype were crossed to males with UAS-shRNA or UAS-ORFs for clonal knockdown or overexpression, respectively.

(A ) qRT-PCR analysis validates reduced levels of Dali in three clonal Dali knockdown cell lines compared to a control line.

Individual clones were selected and tested for PITX2 expression and several PITX2 knockdown clonal lines were established.

In addition, we performed rescue experiments in which human NPHP1 or NPHP4 cDNA was expressed in MDCK knockdown clonal cell lines (Fig.  1E).

Two siRNAs to generate shp38α lentiviral constructs capable of stable p38α knockdown in clonal HLSCs (SH2/SH3).

Two wells of the same clonal line of PITX2 knockdown or non-targeting sequence were pooled and used for RNA extraction as described earlier.

Elegant targeting techniques – including the UAS/GAL4 system, FLP-FRT-mediated recombination and MARCM clonal analysis –enable gene knockdown in specific tissues or patches of cells, bypassing issues of organism lethality.

Similarly, DNMT3B knockdown also reduced clonal ability of human pluripotent stem cells NTERA2 and H7.

Tcf3 knockdown also allowed transient clonal expansion in serum without LIF.

Because all these results were obtained from single clones of Nck1- and Nck2-knockdown cells, we confirmed these findings using polyclonal Nck1- and Nck2-knockdown lines to exclude clonal variations.