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Recovery of colonies was efficient: approximately 1 colony could be isolated from approximately 13 cells deposited (clonal efficiency of 7.7%).
Clonal efficiency was assessed using a limiting dilution assay.
Clonal efficiency was quantified as the cell concentration able to obtain clones development in 50% of wells.
When such adherent serum-stimulated (SS) NS/PCs were returned to serum-free medium with mitogens, they formed floating neurospheres within one week maintaining their clonal efficiency.
We show that clonal expansion is rigidity-dependent, with an optimal clonal efficiency on the 20 kPa.
However, the clonal efficiency of SVZ primary neurospheres while comparable between asymptomatic (e.g. 10 dpi) EAE mice and HC became different once clinical EAE started.
Similar(46)
In the case of negative clonal selection, tumorigenic efficiency does not continue to increase with increased mutation rate, and actually decreases beyond an optimum rate.
Addition of LIF to Esrrb-overexpressing cells increased clonal self-renewal efficiency.
The tumour population was allowed to evolve under this model and the clonal structure and efficiency of tumorigenesis was examined under a range of model parameters.
Furthermore, null cells did not exhibit any overt sign of spontaneous differentiation or appreciable cell death and were able to generate undifferentiated colonies at clonal density with efficiency equal to wild-type cells.
The cell surface sialyl Lewis ligands on cancer cells generated by C2GnT1 expression increase the binding efficiency of clonal cancer cell lines to E-selectin on endothelial cells which is directly proportional to their metastatic potential [39].
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