Sentence examples for clonal densities from inspiring English sources

Filtered cells were counted and directly plated onto polyornithine (1.6 mg/ml ) + fibronectin (2 µg/ml) coated dishes at clonal densities (∼10 cells/cm2).

In order to assess the differentiation potential of emNCSCs at a single cell level, we performed clonal analysis experiments by plating single cell dissociated emNCSCs at clonal densities as previously described by the Studer group[15].

To compare the repulsive properties of different sema3s we seeded HEK293 cells secreting different semaphorins on top of monolayers of human umbilical vein endothelial cells (HUVEC) at clonal densities.

We show by mass spectrometry that nsph-CM contains CSPG, ApoE and cystatin C. The nsph stimulatory effect of nsph-CM can be attributed to CSPG and ApoE but not cystatin C. We show that exogenous CSPG added to NSCs/NPs at clonal densities increases nsph number and size.

Sorted cells were seeded at clonal densities and colonies formed evaluated after two weeks.

With clonal densities of around 100/cm, we estimate a typical separation between clones to be around 100 cell diameters.

Cells were subjected to a pair assay by plating dissociated single cells at clonal density for 12 36 h.

Worthy of note, sphere growth was conducted at clonal density (0.25-0.5 cells/μL) to avoid cell aggregation and sphere fusion.

When seeded at clonal density, CD34+ keratinocytes formed larger colonies than CD34– cells (p<0.05), indicating a higher proliferative potential.

In this new culture system, these human pluripotent stem cells can stably grow, even if in clonal density with keeping expression of stem cell markers.

When plated above clonal density (10 cells/µl) many spheres formed by 1 week (Fig. 1A).