Regarding the 3 CLN harboring metastatic ATC (Table 1, CLN number 10, 12 and 13), FNAB-C identified correctly one of them (CLN number 10) and gave the erroneous diagnosis of metastatic PTC in the remaining two (from the same patient).
This was the case (Table 1, CLN number 19 and 20) of the patient harboring metastatic MTC and characterized by elevated level of serum calcitonin in which FNAB-C indicated metastatic PTC, while on the contrary both FNAB-Tgp and FNAB-Tgm were negative, and FNAB-CTm positive.
Moreover, we predicted 40 mRNAs to be targets of cln-MIR156 family miRNAs (Table 4 and Additional file 7).
Sequence analysis demonstrated α-Syn peptides (yellow highlighted sequences, Figure 1C) in the VMB from both PBS- and MPTP-treated mice but exclusively in the CLN of MPTP-intoxicated mice (Table 1).
As expected, a major disagreement among the three diagnostic tests was observed in the 3 CLN harboring metastasis from ATC (Table 2, CLN number 10, 12 and 13).
Histology showed metastatic papillary TC (PTC) in 26 CLN, metastatic medullary TC (MTC) in 3 CLN, metastatic anaplastic TC (ATC) in 3 CLN and 3 metastatic CLN from extra-thyroidal cancers.
As expected, in these 3 CLN FNAB-Tgp levels were low, only one (Table 1, CLN number 13) being above the cut-off value.
Specificity, sensitivity, PPV, NPV and accuracy are reported in Table 3. Upper panel includes 26 CLN with metastatic PTC and ATC.
In the remaining 5 cases FNAB-C diagnosis of metastatic PTC in 4 CLN was incorrect because the histology demonstrated 2 metastatic MTC (Table 1, CLN n. 19 and 20) and 2 metastatic ATC (Table 1, CLN n. 12 and 13), while the fifth CLN with a benign cytology turned out to be a metastatic PTC (Table 1, CLN n. 34) These 5 FNAB-C diagnoses were considered as false negative.
The results obtained with the GMM-HMM systems trained using the clean data and the multi-condition data (GMM-HMM (cln) and GMM-HMM (mc)) are shown in row 1 and 2 of Table 1, respectively.
