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Using machine-learning algorithms, researchers analyze these video clips to identify discrete temporal "events" by extracting patterns in the different types of shots and the order in which they occur.
Activists learned the hard way during emergency rule that pro-Musharraf officials and security personnel would monitor new media content, particularly Flickr images and YouTube video clips, to identify and arrest protestors and democracy advocates.
After reimplantation of the manipulated embryos, pups were screened for the presence of the transgene by PCR analyses of genomic DNA from tail clips to identify those harboring the transgene DNA.
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Here, we use CLIP to identify RNA targets for the shuttling SR protein, SF2/ASF, in order to test the hypothesis that this SR family protein member regulates nuclear and cytoplasmic expression of specific mRNAs in living cells.
Adult zebrafish at 3 months were fin clipped to identify the genotype as previously described (39).
We employed HITS-CLIP to identify which miRNAs participate in liver regeneration, and to identify their target mRNAs.
We next performed Photoactivatable-Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation (PAR-CLIP) to identify the targets of WTAP and METTL3 proteins.
For example, Chi et al. [ 56] developed a technique, known as high-throughput sequencing of RNA isolated by crosslinking immunoprecipitation (HITS-CLIP), to identify direct miRNA targets.
Here, we employed Argonaute HITS-CLIP to identify miRNA targets in the bovine retina, an animal model used extensively in vision research.
Moreover, the use of HITS-CLIP to identify novel exons that function in regulating transcript levels is a clever application of an already powerful genome-wide approach.
Here we used PAR-CLIP to identify thousands of binding sites of RBM10 and observed significant RBM10 RNA interactions in the vicinity of splice sites.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com