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In C57Bl/6 J mice, DNA was extracted from tail samples, with the exception of C57BL/6 J mouse 2 where ear clip tissue was used.
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Extracardiac tissue is then trimmed off and the aorta clipped just proximal to the arch.
Tissue is 90percentt water.
Scar tissue is stronger.
Fin clips or muscle tissues were obtained from thirty-six individuals representing nine sub-species of cutthroat trout, plus the Bear River cutthroat trout and rainbow trout (including Columbia redband trout and steelhead, which are unique forms of O. mykiss) from the Monte L. Bean Life Science Museum ichthyological collection (Table 1).
If these fragile lymphatic tissues are not clipped or sutured, they remain open and become an important source of free retroperitoneal lymph, setting up the basis for the development of lymphatic complications.
Tissues were collected by toe-clipping, or from voluntary tail autotomy.
A second and third excision of fin tissues were repeated with 5 days intervals to obtain regenerated tissues (fin clip2 and fin clip 3, respectively).
Tissues were taken either from fresh specimens collected in the field or preserved museum specimens, either by toe clips, liver or muscle tissue.
Frogs were collected by hand or with a net, and a tissue sample (toe clip) was taken upon capture.
For genotyping, about 3 5 mm of tail tip (soft tissue only) was clipped using a sterile, sharp scalpel.
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