DNA was isolated from fin-clip tissue samples using the DNeasy Blood & Tissue Kit (Qiagen) and standard protocols, and DNA concentrations were measured with a NanoDrop ND1000 spectrophotometer.
All fin clips or tissue samples were preserved in 95% ethanol (1∶10 w∶ v) upon collection.
For the PCR amplification of genomic DNA from fin clips, the tissue samples were incubated in TE supplemented with 5% Chelex-100 (BioRad Laboratories, Hercules, CA) and 10 µg/ml Proteinase K (Roche, Germany) for 4 hr at 55°C and 10 min at 95°C and then stored at 4°C. 0.1 1 µl of the supernatant was used as template in a standard PCR of 25 µl.
Fin clips or muscle tissue samples were used to extract DNA from all samples.
In summary, fin clips or muscle tissue samples were taken and placed in 95% ethanol.
The parameter estimates (Table 3C) confirmed that the prevalence of Bd was (1) significantly higher for bag rinses and toe clips than for swab tissue samples, (2) significantly higher in the spring and fall than in the summer, and (3) significantly higher in 2005, 2006, and 2007 than in 2003.
Genomic DNA was extracted from tissue samples (fin clips) with a DNA extraction kit DNeasy Tissue Kit (Qiagen GmbH, Hilden, Germany) according to the manufacturers protocol.
Tissue samples (fin clips) were collected from juveniles during 2006 201010 and preserved in ethanol.
Small tissue samples (fin clips) were taken from all adults in the study population to identify parent offspring relationships.
DNA was extracted and isolated from tissue samples (either ear clips from live animals, or a small snip of muscle from dead animals) using the QIAamp DNA Micro Kit (Qiagen, Inc).
Scientists waste time cutting and splicing tissue samples.
