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Within 48 h after alloxan administration, blood glucose concentrations were measured via tail clip sampling.
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That was mostly because the first half was loaded with clip samples and retrospectives, while the latter part moved along with the speed that one of its producers, Bill Mechanic, had promised in advance.
For DLD1 CLIP samples, 83.2% of 17225 peaks were located in exonic regions using distinct reads.
Toe clip samples for genetic analyses were collected under ethics approval granted by the Department of Conservation New Zealand Animal Ethics Committee (permit no. 181).
About 20% of reads could be uniquely mapped to the genome for CLIP samples, while ~60% of reads could be uniquely aligned to the genome for RNAseq samples.
Fin clip samples from 32 individuals were sampled from each of the nine collection sites along the east coast of the United States (Fig. 1; Table 1).
DNA was extracted from fin clip samples with the Qiagen DNeasy 96 Blood & Tissue kit according to the manufacturer's recommendations.
Furthermore, using distinct reads obtained higher percentage of peaks with GGAG motif compared to using all reads, especially for DLD1 and Lovo CLIP samples.
White muscle and/or fin clip samples were collected during fieldwork in Zambia in 2007 and 2008 using a standard operating procedure described in [ 29].
Compared to the method using all reads, using distinct reads yielded fewer peaks but a higher percentage of peaks in exonic regions for all CLIP samples.
A slight increase in the percentage of exonic peaks was observed for Caco-2 CLIP samples using distinct reads versus all reads, while a larger increase was shown for DLD1, Lovo, and mouse colon samples, especially for DLD1 and Colon-2 CLIP samples (Additional File 3).
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