Sentence examples for clip samples were from inspiring English sources
White muscle and/or fin clip samples were collected during fieldwork in Zambia in 2007 and 2008 using a standard operating procedure described in [ 29].
CLIP samples were prepared from Caco-2 cells (three replicates), DLD1, and Lovo cell lines (one replicate each) with a doxycycline-inducible LIN28B and colonic epithelia of Vil-Lin28bMed mice (two replicates) using a modified CLIP-Seq protocol [ 31].
For Alkbh1 genotyping, ear-clip samples were degraded by incubation in PBND buffer (50 mM KCl, 10 mM Tris-HCl pH 8.3, 2.5 mM MgCl2-6H2O, 0.1 mg/ml gelatin, 0.45% v/v NP40, 0.45% v/v Tween 20) and 0.5 mg/ml proteinase K at 55°C over night.
Sequencing libraries from Ago2 HITS-CLIP samples were generated and analyzed using previously described methods (Chi et al., 2009; Moore et al., 2014).
Muscle tissue or fin-clip samples were collected from adult C. gunnari and N. rossii from South Georgia, South Orkney Islands, Elephant Island, and either Dallman Bay (C. gunnari) or South Shetland Islands (N. rossii) (Fig. 1).
The peak amplitude, its time, and the number of clipped samples are reported at the end of processing.
All fin clips or tissue samples were preserved in 95% ethanol (1∶10 w∶ v) upon collection.
Fingernails and toenails were clipped and hair samples were cut.
Fish were sized, and fin clips and muscle samples were preserved in 90% ethanol for DNA analyses.
For the PCR amplification of genomic DNA from fin clips, the tissue samples were incubated in TE supplemented with 5% Chelex-100 (BioRad Laboratories, Hercules, CA) and 10 µg/ml Proteinase K (Roche, Germany) for 4 hr at 55°C and 10 min at 95°C and then stored at 4°C. 0.1 1 µl of the supernatant was used as template in a standard PCR of 25 µl.
