Combining it with immunoprecipitation-based biochemical assays, such as cross-linking immunoprecipitation (CLIP), provides a genome-wide method to detect the sites at which proteins bind DNA or RNA.
Cross-linking the RBP to the RNA using UV radiation before immunoprecipitation (CLIP) provides a way of both ensuring that in vivo contacts are maintained, as well as narrowing down the binding site [ 76].
Home-movie footage and well-sourced TV/movie clips provide a tantalising patchwork upon which the narrative fails to build, with investigation of Altman's methods limited to platitudes about a script being little more than a blueprint, and fleeting descriptions of his sound-recording techniques.
The following clips provide a snapshot of the discussion.
Journal articles, excerpts from classic texts, and some slides and film clips provided a basis for Nadia's lively, informal lectures and for class discussion -- entirely in French.
After SPA identification, studies have indicated that cauterization, rather than clipping, provides a higher success rate.
However, HITS-CLIP provides a powerful means of providing unambiguous and unbiased sites of RNA protein interaction.
Coupling CLIP with next-generation high-throughput sequencing technology, known as CLIP-seq or HITS-CLIP, provides a cost-efficient method to increase the sensitivity of the assay by surveying the RNA landscape on a more global scale.
This technique, known as Argonaute high-throughput sequencing of RNAs isolated by crosslinking immunoprecipitation (HITS-CLIP), provides a "snapshot" of miRNA target interactions in living cells from a given tissue.
These clips provided a basis for facilitated discussions related to living with long-term conditions, understanding patients and their context, and the nature, make-up, and role of health care teams.
