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In vivo studies using tail clip method indicated that FVIII cochleate complex is effective and protects hemophilic mice from bleeding.
Fully expanded and healthy rice leaves were inoculated approximately 2 cm from the leaf tip using the scissors- clip method (Chern et al. 2005).
oryzae race PXO99 (Xoo) using the leaf clip method [45].
Thus we feel the CLIP method has captured a subset of biologically relevant SF2/ASF binding sites.
The cross-linking immunoprecipitation (CLIP) method is a powerful method for elucidating the target specificity of RNA binding proteins in vivo [12].
The CLIP method revealed that a subset of SF2/ASF mRNA targets, including PABP, NETO2, ENSA and SFRS1, were bound by SF2/ASF in the cytoplasm of HEK293T cells.
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Ultraviolet (UV) CLIP methods [ 22, 31 33] overcome some drawbacks of SELEX and RIP-CHIP.
Nonetheless, HITS-CLIP and CLIP methods discussed above cannot achieve individual-nucleotide resolution of the binding site.
Other methods, such as mass spectrometry, can also be used for characterization of cross-linked RBPs and RNAs instead of CLIP methods.
If the data have been generated by CLIP methods without the use of photoactivatable nucleosides, the read coverage can be used as a criterion of the cluster quality.
Variable crosslinking efficiency also explains why many top predictions of the context++ model are missed by the CLIP methods, as indicated by the modest overlap in the CLIP identified targets and the top predictions.
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