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Most importantly, the HLP1 binding targets from the two sets of HLP1-CLIP libraries share significant overlap (P = 0, Supplementary information, Figure S6C), suggesting the reproducibility of the CLIP experiment.
AM constructed plasmids and assisted in CLIP experiment.
For each of the clusters, the number of CLIP tags for each nuclear and cytoplasmic CLIP experiment was counted.
In a typical CLIP experiment, extracts are made from cells after UV-irradiation and treated with RNase to retain only the RNA region protected by the interacting protein.
Algorithmic improvements of different analysis stages, such as read alignment or functional annotation and analysis, may allow us to more fully assess the outcomes of a CLIP experiment.
6) The CLIP experiment was done with over-expressed RBM47 protein and this has the potential to influence the observed binding sites.
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Cells were harvested and CLIP experiments were performed using either anti-AGO family or elF2C antibodies as described (28).
BC was calculated for nuclear and cytoplasmic CLIP experiments, respectively.
The second subset considered all the other CLIP experiments, namely CLIP, CLIP-seq, HITS-CLIP and iCLIP.
The other two plots are similar but the correlations were calculated using two Nuc CLIP experiments or two Cyt CLIP experiments.
AR performed LARS2 CLIP experiments and preparation of these samples for ion torrent sequencing.
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