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When available, target genes identified using high-throughput CLIP data were collected from the supplemental materials of the corresponding studies (Lipchina et al., 2011; Loeb et al., 2012; Helwak et al., 2013; Grosswendt et al., 2014).
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GO analyses of DEGs and PAR-CLIP data were performed using DAVID.
55% of the proteins with miRNA targets sites predicted based on PAR-CLIP data were moderately down-regulated (log2-fold change < -0.1).
As a result of the study, a set of background binding events in PAR-CLIP data is publicly available in GEO (GSE50989).
This implies miRNA targets identified from PAR-CLIP data are more likely to be in a protein complex from the CORUM database (12%) as compared to proteins in general (2%).
The parameters used for the OM-OFDM transmission are given in Table 5. OM-OFDM, OFDM, and clipped OFDM data were sent through a 5-tap typical-urban area by using the parameters previously mentioned.
The RBPs corresponding to these CLIP-seq data were further annotated with detailed information that was manually curated from other databases, such as NCBI [ 25], CISBP-RNA [ 26] and RBPDB [ 27].
The experimental manipulations were induced using video clips and the data were analyzed using a repeated measures MANOVA procedure.
Argonaute (AGO) binding-site clusters in mouse determined from the mapped CLIP-Seq experimental data were taken from StarBase (19).
All spatial data were clipped to the study area boundary.
The vector sequences of the base-called EST data were clipped.
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