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Deacetylase candidates identified by clip-chip were overexpressed and purified in E. coli.
This study also highlights several advantages of the clip-chip approach.
In the future, the clip-chip approach could be used to discover new members of other enzyme families.
Finally, the clip-chip approach is capable of functional annotation of enzymes using both gain- and loss-of-signal reactions.
Now, Tu, Guo, Chen et al. have devised a technique to identify new deacetylases, called the "clip-chip" approach.
Second, the clip-chip approach does not require any prior knowledge of the enzymes of interest as long as a robust biochemical assay is available.
DOI: http://dx.doi.org/10.7554/eLife.05322.015 In this study, we have applied a clip-chip approach to identify new KDAC candidates in E. coli.
The paper describes an interesting study that uses an application of proteome array "Clip-Chip" technology to the discovery of new enzymatic functions.
The principle behind the clip-chip approach is the delivery of thousands of purified proteins spotted on a glass slide (e.g., a proteome microarray) to a substrate of interest immobilized on another slide (i.e., the substrate slide) such that thousands of desired biochemical reactions can be carried out in parallel, in order to identify new enzymes of interest.
Gather building supplies including, large clips such as chip clips or hair clips to use as a securing device once you've built the fort.
Get two clips (i.e clothespins, chip clips, detachable cell phone clips, etc. ) and clip the bottom corners into place.
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