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Serum total cholesterol and triglycerides were determined enzymatically (22, 23), and HDL cholesterol was determined using a modified precipitation technique (24) based on the lipid research clinics method (25).
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At high SAA concentrations the CV of the in-clinic method for inter-assay variation was 12%, which is acceptable for clinical purposes considering the biological behaviour of SAA.
The stability of SAA with the in-clinic method was not evaluated.
Intra- and inter- assay variation of the in-clinic method was determined.
Only 11 samples (18%) had SAA concentrations of 10-270 mg/L with the in-clinic method.
The in-clinic method was previously reported to have an acceptable linearity under dilution [ 17].
The in-clinic method (EVA1) results were compared to a reference method (Eiken LZ SAA) with 62 patient samples.
The imprecision of the in-clinic method was acceptable at high SAA concentrations but not at low concentrations.
One sample had a SAA concentration of <10 mg/L with the in-clinic method and 19 mg/L with the reference method.
We remunerated each group practice, minimum 3 physicians with a focus on obstetrics, participating in the fax or in-clinic method (see following) $250/month.
It is recommended that each laboratory using the in-clinic method evaluate their own imprecision at different concentrations, and also determine reference values and cut off values.
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