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Although the generation of different mature hematopoietic cell types from PSC has been proven successful using classical two-dimensional (2D) differentiation cultures4,10,11,12,13, these systems do not allow for the generation of iPSC-derived cells in clinically relevant quantities.
"As long as you are working on your mouse or in vitro models," writes stem cell consultant Reinout Hesselink, "that is no problem". But this approach is not feasible to prep cells in clinically relevant quantities (millions and billions) that are necessary for patients when the time comes.
A gene transfer vector should be safe, stable, cost-effective to manufacture in clinically relevant quantities, and capable of efficient and tissue-specific delivery.
Scientific literature on depression and anxiety in patients with coronary heart disease (CHD) consistently reports data of elevated anxiety and depression scores indicating clinically relevant quantities of these psychopathological conditions.
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Computational simulations can now produce clinically relevant hemodynamic quantities using only non-invasive measurements, combining detailed three dimensional fluid mechanics with physiological models in a multiscale framework.
Many attempts have been made to develop techniques that allow production of large quantities of clinically relevant islet β-cells in vitro.
For example, the series of DREAM challenges empowered the global scientific community to build application-specific, clinically relevant predictive biological networks using vast quantities of genomic data.
This method was specifically designed to increase the quantities of DNA in clinically relevant samples such as single cell analysis, pre-implantation genetic diagnosis and forensic typing [ 6].
These measured quantities were found to be clinically relevant and are particularly promising as biomarkers for breast cancer diagnosis [ 11– 15].
However, the reason for exclusion of 108 patients was technical (DNA sample quality and quantity) and not linked to any clinically relevant variables.
From this additional information, the time-varying probability distributions of the ICP waveform can be extracted, and it is these latter quantities that enable computation of the probabilities of clinically relevant events.
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