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5 In 2010, WHO issued guidance to resource-constrained settings to expand the eligibility of the treated population, by recommending initiation of ART when a patient's CD4 T-cell count reached 350/μL or less, or was clinically necessitated.
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Treating excitotoxicity using glutamate-receptor antagonists has not proven clinically viable, necessitating more sophisticated approaches.
We were interested in developing a clinically relevant predictive gene set, which necessitated a reduction in the number of genes in the predictor, since a large number of genes is prohibitive for clinical assays such as quantitative rt-PCR.
The International Classification of Headache Disorders version 2 was applied and as many diagnoses as necessitated by the criteria and as clinically justified were assigned to each patient[3].
Additionally, clinically relevant genes were excluded due to severe multiple testing correction necessitated when using the 10 Mb symmetrical window, including HBG1, which is a hemoglobin subunit very closely related to HBG2.
These realities combined with increasing evidence of the lack of efficacy of the clinically assessed ABCDE criteria ("A" for "asymmetry," "B" for "border irregularity," "C" for "color variation," "D" for "diameter," and "E" for "evolving lesions") have necessitated ongoing efforts to enhance the earlier clinical detection of melanoma [3, 4].
That gap necessitated some juggling.
Their weight necessitated structural reinforcement.
My excitement just necessitated it.
The economy has necessitated that.
War damage necessitated their reconstruction after 1945.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com