Exact(4)
The sponge stopper treatment had no measurable effect on flies' climbing response, as flies kept off the machine with a stopper had climbing indexes that were not significantly different from flies kept off the machine without a stopper (multivariate regression, stopper effect, p = 0.3018, Fig. 2C).
The flight circuit identified here appears similar to the one identified recently for the startle induced climbing response which requires PAM dopaminergic inputs to the β′ lobe (Riemensperger et al., 2013).
Young males expressing dGS-RNAi displayed a normal climbing response, with no significant change in climbing speed relative to GFP-expressing control flies (Fig. 6C).
However, attempts to induce a similar novelty/anxiety response by changing the colour of the environment did not provoke similar climbing behaviour (data not reported), and so it is not clear why such a clear climbing response was elicited.
Similar(56)
Various functions have been suggested for BK channels [6], including climbing fiber response modulation [2].
Climbing fiber responses can be readily recorded during motor performance [58], [59], [60], [61].
DOI: http://dx.doi.org/10.7554/eLife.02076.004 10.7554/eLiFigure76.005 Figure 2 figure supplement 1. Climbing fiber responses during stimuli in the 'off' direction.
Therefore in the current paper, we paired stimulation experiments with highly complementary recording experiments, which showed that the natural climbing fiber responses present during visual-vestibular VOR-decrease training are not correlated with the induction of plasticity either.
Therefore in the current manuscript, we pair the stimulation experiments with highly complementary recording experiments, which show that the natural climbing fiber responses present during visual-vestibular VOR- decrease training are not correlated with the induction of plasticity either.
For example, d3 and d4 could both evoke non-CF signals below threshold for supra-linearity, but d4 could generate climbing fiber responses with a higher number of spikes in a larger fraction of the trials.
Climbing fiber responses are plotted as the probability of a spike in each in 50 ms bin, as in Figure 2E,F (G and H ) Purkinje cell simple spike firing rate.
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