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The German government recently set up a "climate Cabinet" to coordinate efforts to combat climate change.
Germinated seeds were grown in pots with soil in an illuminated climate cabinet at 30°C.
To accelerate the measurement, the tests were performed in a climate cabinet (Binder KBF 115, BINDER GmbH, Tuttlingen, Germany) at 60℃and 90% relative humidity (RH).
Note that heating rates may differ between the water bath and the climate cabinet.
This, however, does not confound any result shown, as always the same method (either water bath or climate cabinet) was used within one experiment.
To determine heat knock-down time, butterflies were placed in small, sealed glass vials (40 ml), which were submerged in a water bath or transferred to a climate cabinet (Sanyo MIR-553), both set at a constant temperature of 45°C (again in a randomized block design).
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Strong wild type seedlings approximately 3 weeks old were transferred from natural fields to artificial climate cabinets (MMM, Climacell, Germany).
After 22 days in artificial climate cabinets, RNA was isolated from the leaves once every 3 h for 24 h using Trizol solution (Invitrogen, USA).
Two photoperiods were used in the artificial climate cabinets with the following parameters: Long-day condition (LD), 15 h light and 9 h dark at 28 °C; short-day condition (SD), 9 h light and 15 h dark at 28 °C.
Therefore, we used six climate cabinets (Sanyo MLR-351H) set at 27°C, 70% relative humidity, and a photoperiod of L12 D12.
Measurements were performed with a portable porometer (HCM 1000; Heinz Walz GmbH, Effeltrich, Germany), which was placed in the climate cabinets.
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