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We then implemented a click methodology to graft the bifunctional ligand to azide-containing glucoside and maltoside scaffolds.
The incorporation of the RNA precursor 5-ethynyluridine (EU) was detected by the "click" methodology (42), using the protocol and the reagents provided by Invitrogen/Molecular Probes (Carlsbad, CA).
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Click, click, click, click, click.
The NMP and "click chemistry" methodology affords polymers with precisely defined number average molecular weights (Mn) ranging from ~ 5500 to 24,000 g mol−1 with a high efficiency for the click grafting of Ru chromophores to the polymer backbone.
In this work, DCs were cultured on surfaces presenting a uniform gradient of the integrin-targeting RGD peptide generated using the recently established "universal gradient substrate for click biofunctionalization" methodology.
It has been shown that DCs cultured on surfaces which presented integrin-targeting RGD peptide were created using "universal gradient substrate for click biofunctionalization" methodology.
As it is evident from the data shown in Figure 5, incorporation of the RNA precursor EU, detected by the "click" chemistry methodology (42), was suppressed by 52 and 86% in cells treated with 2 and 20 nM Mxt, respectively.
Taken together, these results demonstrate a convenient click chemistry methodology that can be applied to identify other metal or covalent modification-based drug targets and suggest a ribotoxic mechanism for cisplatin cytotoxicity.
Click on the "Methodology" section of his website and it says: "This page is being updated - please try later".
(Click here for methodology).
(For the full selection methodology, click here).
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