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By changing the O linkage of the fluorophore-tagged xyloside to click linkage, the fluorophore-tagged click-xylosides were predicted to prime both HS and CS with higher MW chains as many other click-xylosides.
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Click, click, click, click, click.
Meanwhile, accessible CD cavity was determined and the amount of covalently immobilized BSA protein was also quantified after the respective "click" linkages of per-azido-β-CD and BSA-N3 on PMBAA-PET surface were established.
The oxime, maleimide/thiol, and click linkages can be used with more complex molecules, where the conjugation only occurs at specific sites.
Click, click, click.
Here, 33-ϕ-OEG-lipid and 33-OEG-lipid possess the same trisaccharide and differ only in the linkage: the bulkier click-linkage to OEG in the former or the peptide linkage to OEG in the latter.
To watch her exclusive performance of "Soap," exclusive A-Sides interview, and this week's "Delve Into Twelve" countdown, click this linkage.
Copper-free click chemistry allowed linkage of the terminal sugar of the OAg chain to CRM197 even when few or only one linker were present on the protein (Table S1).
One of the most promising future labeling methods for high-density labeling is the 1,2,3-triazole linkage (click chemistry) between a fluorophore modified as an azide and a reaction partner (e.g., protein) modified as an alkyne, or vice versa (Laughlin et al. 2008).
By employing the traceless linker, the designed oligosaccharides can be obtained in pure form and, more importantly, ready for incorporation to aglycons of interest via 'Click' chemistry or amide linkage.
Clicking on the related linkage left column leads to the PubMed search page, as shown in the right column.
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