Your English writing platform
Discover LudwigExact(8)
Of particular interest is the outreach for inverse Click labeling patterns that would create significantly more flexibility for complex labeling patterns as, for instance, needed for single-molecule fluorescence resonance energy transfer (FRET) studies.
'Click' labeling afforded [18F]16 in 37.0 ± 3.6% decay corrected radiochemical yield based on azide [18F]14 and 7% end of synthesis (EOS) yield from aqueous fluoride.
Click labeling on live detached merosomes also showed distinct propargylcholine staining of individual merozoites.
We previously reported an azide-functionalized compound, picazoplatin, designed for post-treatment click labeling that enables detection of Pt complexes (White et al., 2013).
Maschauer et al. combined the click labeling approach with the introduction of sugar derivatives allowing labeling as well as pharmacokinetic optimization in one step [ 32, 75, 76].
It was concluded that the click labeling resulted in comparable yields as found for the fluorobenzaldehyde approach without the need for purification of the prosthetic group.
Similar(52)
At 4.5 hr p.i., cells were fixed, click labeled, immunostained with antiserum against HIV-1 CA, and analyzed.
Beads were washed twice with ice-cold RIPA buffer, twice with 0.1% (w/v) SDS/PBS then resuspended in 50 μl PBS for on-bead click labelling.
a, Schematic of tagged sites in HIV-1JR-FL Env that were used for enzymatic labelling and amber stop codon (TAG -suppressed incorporaTAG -suppressedal amincorporation click labelling.
To this end, we sequentially diluted Alexa Fluor 647 azide (the dye used for EdU click labelling) from 3.5 mM to 3.5 nM and imaged it in the SDCM under the same conditions of detection as used for RTC/PIC.
They showed that the F-click labeling reaction was more convenient by using the β-anomeric derivative of the azides, respectively, alkynes, giving very high RCY of 71±10%0%.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com