Sentence examples for cleaving with from inspiring English sources

He toddles back and forth between the pit and a steel work table streaked with honey-colored grease, cleaving with his right hand and placing portions into plastic foam containers with his bare left.

When required for the pull-down assays, GST was removed by cleaving with Pre-Scission protease (Amersham Biosciences).

The fragment was inserted into the TopoII (Invitrogen) vector; antisense probe was made by cleaving with XbaI and transcribing with SP6 RNA polymerase.

The DNA and the bound proteins are released from the beads by cleaving with a restriction enzyme specific for a recognition sequence included into the bait oligo sequence.

The polyhistidine tag was released from the fusion protein by cleaving with thrombin (Sigma) (2 U/40 μg of fusion protein) for 16 hours at room temperature.

Synaptobrevin and SNAP25 were purified by affinity chromatography using glutathione-agarose beads (Sigma) by cleaving with thrombin in cleavage buffer (50 mM Tris-HCl, 150 mM NaCl, pH 8.0) for 1 hour at room temperature.

The resulting PCR-product was cleaved with NcoI- XbaI and ligated into the NcoI- XbaI-cleaved pBAD24 plasmid.

LONGS PEAK, the highest mountain in Rocky Mountain National Park, looks like it was cleaved with a chisel.

The S−S bond can also be cleaved with alkyllithiums and other organometallic compounds to form sulfides.

The Boc group was later cleaved with 4 M HCl in dioxane.

Cells were then cleaved with TrypLE and counted using a hemocytometer, as described earlier.