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Following the Me-RDA experimental design, Tester DNA was cleaved with FspBI and Driver DNA was cleaved with BfaI (see method, Figure 1A).
The GST tag was cleaved with PreScission protease at 4 °C overnight.
The His-tag (Tat) and His-GB1-tag (AFF) were cleaved with TEV protease.
Crosslinked DNA was cleaved with 1 M pyrrolidine (Sigma) at 90 °C for 20 min.
HOPG substrates were cleaved with scotch tape just prior to loading in the furnace.
LONGS PEAK, the highest mountain in Rocky Mountain National Park, looks like it was cleaved with a chisel.
Isolated, labeled alpha subunit was cleaved with CNBr, and the fragments were separated by reverse-phase high-performance liquid chromatography.
The sample cleaved with PreScission protease was dialysed against 50 mM sodium acetate pH 5.5, 100 mM NaCl.
After trx-PT-Fc was cleaved with recombinant enterokinase, PT-Fc was obtained.
All 54 PCR products having >95·7% similarity to streptomycete sequences were cleaved with Bst YI.
The His-tag was cleaved with TEV protease, and the tag-less toxin A chain was purified on a HiPrep 26/60 Sephacryl S-200 column.
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