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Based on this rationale, they synthesized a short peptide of TPLK|SPPPSPR ("|" is the potentially cleaved position), which was experimentally verified to be a superior substrate of calpain [16].
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(B ) Chromosome 3 profile, where the position of the centromere and of the most frequently cleaved nucleotide position on the chromosome are indicated.
Firstly, Miggin et al. [29] have shown that Mal is cleaved at position 198 by the cysteine protease caspase 1 and that this processing is required for Mal to function in signalling by both TLR2 and TLR4.
Accordingly, N-terminal sequencing performed on the secreted isoform of ElpS-3Flag indicates that the protein is cleaved at position 38 downstream the predicted fatty-acylated cysteine (Fig. 6B).
Peak c could represent a new A-Beta aggregation state or a peptide cleaved after position 5. Mass spectrometry analysis of this fraction showed a peak of 3094 Da (Figure 2B), suggesting another cleaving site between aminoacids 27 28.
(E ) Expansion of the region around the most frequently cleaved nucleotide position at the same scale as in (D ).
(G ) Same as (A ) for the most frequently cleaved nucleotide position on each of the 16 chromosomes.
(B ) The 1-kb interval around the most frequently cleaved nucleotide position on Chromosome 3. (C ) Same as (A ) for all 16 aligned mid-Cens.
RasGAP is a substrate of caspase; when cleaved at position 455, N-terminal fragment of the production protects cells from apoptosis in a Ras/PI3K/Akt-dependent manner.
The gB polypeptide, composed of 906 amino acids, is translated into a 160-kDa precursor molecule that is cleaved at position 460 by cellular endoprotease to produce the N- and C- terminal products [ 11, 13, 14].
Repeat-derived piRNAs are thought to be generated via a "ping-pong" mechanism [ 17]: a piRNA hybridizes to a target, which is subsequently cleaved along position 10 of the piRNA.
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